Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16713
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1O2Y5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone Deacetylase 8/HDAC8 (Q9BY41). MEEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Western Blot: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]
Western Blot: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Analysis of extracts of various cell lines, using Histone Deacetylase 8/HDAC8 Rabbit mAb (NBP3-16713) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]
Immunocytochemistry/Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Immunofluorescence analysis of NIH-3T3 cells using Histone Deacetylase 8/HDAC8 Rabbit mAb (NBP3-16713) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Mouse testis using Histone Deacetylase 8/HDAC8 Rabbit mAb (NBP3-16713) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone Deacetylase 8/HDAC8
Alternate Names
HDAC8, HDACL1, RPD3
Gene Symbol
HDAC8
Additional Histone Deacetylase 8/HDAC8 Products
Product Documents for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Product Specific Notices for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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