Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16713

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16713-100ul
NBP3-16713-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1O2Y5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone Deacetylase 8/HDAC8 Antibodies »

Product Summary for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone Deacetylase 8/HDAC8 (Q9BY41). MEEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Analysis of extracts of various cell lines, using Histone Deacetylase 8/HDAC8 Rabbit mAb (NBP3-16713) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Immunofluorescence analysis of NIH-3T3 cells using Histone Deacetylase 8/HDAC8 Rabbit mAb (NBP3-16713) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Mouse testis using Histone Deacetylase 8/HDAC8 Rabbit mAb (NBP3-16713) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 8/HDAC8

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.

Alternate Names

HDAC8, HDACL1, RPD3

Gene Symbol

HDAC8

Additional Histone Deacetylase 8/HDAC8 Products

Product Documents for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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