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Histone H1.1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00003024-B01P

Novus Biologicals, part of Bio-Techne
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H00003024-B01P

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HIST1H1A (NP_005316.1, 1 a.a. - 215 a.a.) full-length human protein. MSETVPPAPAASAAPEKPLAGKKAKKPAKAAAASKKKPAGPSVSELIVQAASSSKERGGVSLAALKKALAAAGYDVEKNNSRIKLGIKSLVSKGTLVQTKGTGASGSFKLNKKASSVETKPGASKVATKTKATGASKKLKKATGASKKSVKTPKKAKKPAATRKSSKNPKKPKTVKPKKVAKSPAKAKAVKPKAAKARVTKPKTAKPKKAAPKKK

Specificity

HIST1H1A - histone cluster 1, H1a,

Marker

Nuclear Marker

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for Histone H1.1 Antibody

Western Blot: Histone H1.1 Antibody [H00003024-B01P]

Western Blot: Histone H1.1 Antibody [H00003024-B01P]

Western Blot: Histone H1.1 Antibody [H00003024-B01P] - Analysis of HIST1H1A expression in transfected 293T cell line by HIST1H1A polyclonal antibody. Lane 1: HIST1H1A transfected lysate(23.65 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Histone H1.1 Antibody [H00003024-B01P]

Immunocytochemistry/ Immunofluorescence: Histone H1.1 Antibody [H00003024-B01P]

Immunocytochemistry/Immunofluorescence: Histone H1.1 Antibody [H00003024-B01P] - Analysis of purified antibody to HIST1H1A on HeLa cell. (antibody concentration 10 ug/ml)

Applications for Histone H1.1 Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone H1.1

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Alternate Names

H1 histone family, member 1, H1.1, H1A, H1F1HIST1, histone 1, H1a, histone cluster 1, H1a, histone H1.1, MGC126642, MGC138345

Entrez Gene IDs

3024 (Human)

Gene Symbol

H1-1

Additional Histone H1.1 Products

Product Documents for Histone H1.1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone H1.1 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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