Histone H2a [p Ser129] Antibody (SR1183)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21526

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21526-100ul
NBP3-21526-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Yeast

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1183

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H2A Antibodies »

Product Summary for Histone H2a [p Ser129] Antibody (SR1183)

Immunogen

A synthesized peptide derived from yeast phospho-Histone H2a [Ser129] (Uniprot #: P04912)

Modification

p Ser 129

Specificity

Detects endogenous levels of total phospho-Histone H2a [Ser129]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H2a [p Ser129] Antibody (SR1183)

Western Blot: Histone H2a [p Ser 129] Antibody (SR1183) [NBP3-21526] -

Western Blot: Histone H2a [p Ser 129] Antibody (SR1183) [NBP3-21526] - Western blot analysis using NBP3-21526 in Saccharomyces cerevisiae treated with Methyl methanesulfonate.

Applications for Histone H2a [p Ser129] Antibody (SR1183)

Application
Recommended Usage

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2A

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).

Long Name

H2A Histone Family

Alternate Names

H2A1B, HIST1H2AB

Gene Symbol

H2AC20

Additional Histone H2A Products

Product Documents for Histone H2a [p Ser129] Antibody (SR1183)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H2a [p Ser129] Antibody (SR1183)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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