Histone H2AE [p Ser1] Antibody (RM216)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61489
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Vertebrate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM216
Concentration
1 mg/ml
Product Specifications
Immunogen
An unmodified peptide corresponding to the N-terminus of Histone H2AE
Modification
p Ser1
Specificity
This antibody reacts to Histone H4 or Histone H2AE phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2AE [p Ser1] Antibody (RM216)
Western Blot: Histone H2AE [p Ser1] Antibody (RM216) [NBP2-61489] -
Western Blot: Histone H2AE [p Ser1] Antibody (RM216) [NBP2-61489] - Western Blot of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole. Using NBP2-61489 at 0.5 ug/ml, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.
Immunocytochemistry/Immunofluorescence: Histone H2AE [p Ser1] Antibody (RM216) [NBP2-61489] - Immunocytochemistry of HeLa cells, using NBP2-61489 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Multiplex Immunoassay: Histone H2AE [p Ser1] Antibody (RM216) [NBP2-61489] - NBP2-61489 specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).
Applications for Histone H2AE [p Ser1] Antibody (RM216)
Application
Recommended Usage
ELISA
0.2 ug/ml - 1 ug/ml
Immunocytochemistry/ Immunofluorescence
1 ug/ml - 2 ug/ml
Multiplex Immunoassay
0.1 ug/ml - 1 ug/ml
Western Blot
0.5 ug/ml-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2AE
Alternate Names
H2A histone family, member M, H2A/m, H2AFA, H2AFMHistone H2A/m, HIST1H2AE, histone 1, H2ab, histone cluster 1, H2ab, histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a
Gene Symbol
H2AC8
Additional Histone H2AE Products
Product Documents for Histone H2AE [p Ser1] Antibody (RM216)
Product Specific Notices for Histone H2AE [p Ser1] Antibody (RM216)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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