Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26017
Recombinant Monoclonal Antibody
![Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26017_rabbit-histone-h2ae-mab-rm216-azide-and-bsa-free-22620242217442.png "Immunocytochemistry/ Immunofluorescence: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] -")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Vertebrate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM216
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free
Immunogen
An unmodified peptide corresponding to the N-terminus of Histone H2AE
Specificity
This antibody reacts to Histone H4 or Histone H2AE phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] -
Immunocytochemistry/ Immunofluorescence: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] - Immunocytochemistry of HeLa cells, using Clone RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).![Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26017_rabbit-histone-h2ae-mab-rm216-azide-and-bsa-free-22620242311071.png "Western Blot: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] -")
Western Blot: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] -
Western Blot: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] - Western Blot of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole, using RM216 at 0.5 ug/mL, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.![Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26017_rabbit-histone-h2ae-mab-rm216-azide-and-bsa-free-22620242311075.png "Multiplex Immunoassay: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] -")
Multiplex Immunoassay: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] -
Multiplex Immunoassay: Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free [NBP3-26017] - RM216 specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).Applications for Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.2 ug/ml - 1 ug/ml
Immunocytochemistry/ Immunofluorescence
1 ug/ml - 2 ug/ml
Multiplex Immunoassay
0.1 ug/ml - 1 ug/ml
Western Blot
0.5 ug/ml-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2AE
Alternate Names
H2A histone family, member M, H2A/m, H2AFA, H2AFMHistone H2A/m, HIST1H2AE, histone 1, H2ab, histone cluster 1, H2ab, histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a
Gene Symbol
H2AC8
Additional Histone H2AE Products
Product Documents for Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free
Product Specific Notices for Histone H2AE [p Ser1] Antibody (RM216) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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