Histone H2AE Antibody (RM225)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61490

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Vertebrate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM225

Concentration

1 mg/ml

View all Histone H2AE Antibodies »

Product Summary for Histone H2AE Antibody (RM225)

Immunogen

A peptide corresponding to the C-terminus of Human Histone H2AE

Specificity

This antibody reacts to the Histone H2AE protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2AE Antibody (RM225)

Western Blot: Histone H2AE Antibody (RM225) [NBP2-61490] -

Western Blot: Histone H2AE Antibody (RM225) [NBP2-61490] - Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using NBP2-61490 at 0.5 ug/ml.

Immunocytochemistry/Immunofluorescence: Histone H2AE Antibody (RM225) [NBP2-61490] -

Immunocytochemistry/Immunofluorescence: Histone H2AE Antibody (RM225) [NBP2-61490] - Immunocytochemistry of HeLa cells, using NBP2-61490 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Applications for Histone H2AE Antibody (RM225)

Application
Recommended Usage

ELISA

0.2 ug/ml - 1 ug/ml

Immunocytochemistry/ Immunofluorescence

1 ug/ml - 2 ug/ml

Multiplex Immunoassay

0.2 ug/ml - 1 ug/ml

Western Blot

0.5 ug/ml - 2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2AE

Histone H2AE is 124 amino acids long, weighing approximately 13 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes and is a core component of the nucleosome. Current studies are being done on several diseases and disorders including riddle syndrome, systemic lupus erythematosus, lupus srythematosus, and immunodeficiency. This protein has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the proteasome degradation, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Alternate Names

H2A histone family, member M, H2A/m, H2AFA, H2AFMHistone H2A/m, HIST1H2AE, histone 1, H2ab, histone cluster 1, H2ab, histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a

Gene Symbol

H2AC8

Additional Histone H2AE Products

Product Documents for Histone H2AE Antibody (RM225)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H2AE Antibody (RM225)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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