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Histone H2AX Antibody (CL5157) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44279

Novus Biologicals, part of Bio-Techne
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NBP3-44279

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # CL5157

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P16104, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Histone H2AX Antibody (CL5157) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

: 2-10 ug/ml

Immunohistochemistry

: 1:20000 - 1:50000

Immunohistochemistry-Paraffin

: 1:20000 - 1:50000
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2AX

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody (CL5157) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone H2AX Antibody (CL5157) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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