Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody

Chromatin Immunoprecipitation Sequencing: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689] - Figure 1. ChIP results obtained with the antibody directed against Histone H2A.Z ChIP assays were performed using HeLa cells, the antibody against Histone H2A.Z and optimized primer pairs for qPCR. ChIP was performed on sheared chromatin from 100,000 K562 cells using the iDeal ChIP-seq kit. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed using primers specific for the promoter of the EIF4A2 gene, used as positive control target and for the coding region of the MYT1 gene, and the Sat2 satellite repeat, used as negative control targets. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).

ELISA: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689]

ELISA: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689] - Figure 3. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against Histone H2A.Z . The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the purified antibody was estimated to be 1:265,000.

Dot Blot: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689] - Figure 4. Cross reactivity test using the antibody directed against Histone H2A.Z A Dot Blot analysis was performed to test the cross reactivity of the antibody against Histone H2A.Z with peptides containing other histone acetylations and the unmodified H2A.Z sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 4 shows a high specificity of the antibody for the modification of interest.