Histone H2B, member S Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56347
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Chicken, Drosophila, Mammal, Zebrafish
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunoblotting, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSS of human Histone H2B; GenBank no. CAI19747.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2B, member S Antibody
Western Blot: Histone H2B, member S Antibody [NB100-56347]
Western Blot: Histone H2B, member S Antibody [NB100-56347] - Doxycycline-inducible stable cell lines expressing TDP43 variants. Immunoblotting of whole-cell lysates and cytoplasmic and nuclear fractions for alpha-tubulin (cytoplasmic marker; top panel), H2B (nuclear marker; middle panel), and eGFP (a marker for expression of the eGFP-His-tagged doxycycline-inducible plasmids) following subcellular fractionation of the control. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fncel.2020.00117/full), licensed under a CC-BY license.Immunohistochemistry-Paraffin: Histone H2B, member S Antibody [NB100-56347]
Immunohistochemistry-Paraffin: Histone H2B, member S Antibody [NB100-56347] - Human heart stained with Histone H2B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Western Blot: Histone H2B, member S Antibody [NB100-56347]
Western Blot: Histone H2B, member S Antibody [NB100-56347] - Analysis of Histone H2B using NB100-56347 at 1:250 in nuclear and cytoplasmic fractions made from Drosophila head extracts using Novus' Nuclear Extraction Kit. Data courtesy of Dr. Jerry Yin, University of Wisconsin-Madison.Applications for Histone H2B, member S Antibody
Application
Recommended Usage
Immunoblotting
reported in scientific literature (PMID 28235947)
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
2 ug/ml
Application Notes
A 15 kDa band is observed. This antibody is routinely quality controlled in human lysates.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H2B, member S
Alternate Names
H2B histone family S member10H2B/s, H2B histone family, member S, Histone H2B.s
Entrez Gene IDs
295278 (Rat)
Gene Symbol
H2BFS
Additional Histone H2B, member S Products
Product Documents for Histone H2B, member S Antibody
Product Specific Notices for Histone H2B, member S Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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