Histone H2B type 1B [ac Lys20] Antibody (21F11)

Western Blot: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30 minutes. All lanes: Histone H2B type 1B antibody at 0.135ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa. Observed band size: 15 KDa.

Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30 minutes) with at Histone H2B type 1B antibody 1:84, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Overlay histogram showing Hela cells stained with Histone H2B type 1B antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.