Histone H2B type 1B [ac Lys20] Antibody (RM235)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61496

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-61496
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Vertebrate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM235

Concentration

1 mg/ml

View all Histone H2B type 1B Antibodies »

Product Specifications

Immunogen

An acetyl-peptide corresponding to Histone H2B type 1B [ac Lys20]

Modification

ac Lys20

Specificity

This antibody reacts to Histone H2B type 1B acetylated at Lysine 20. No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2B type 1B [ac Lys20] Antibody (RM235)

Western Blot: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] -

Western Blot: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61496 at 0.5 ug/ml
Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using NBP2-61496 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Multiplex Immunoassay: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - NBP2-61496 specifically reacts to Histone H2B type 1B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

Applications for Histone H2B type 1B [ac Lys20] Antibody (RM235)

Application
Recommended Usage

ELISA

0.2 ug/mL-1 ug/mL

Immunocytochemistry/ Immunofluorescence

1 ug/ml - 2 ug/ml

Multiplex Immunoassay

0.1 ug/ml - 0.5 ug/ml

Western Blot

0.5 ug/ml - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2B type 1B

The HIST1H2BB gene encodes a 126 amino acid long, 13 kDA histone H2B type 1-B that is critical in the nucleosome by functioning in transcription regulation, DNA replication and repair, as well as chromosomal stability. It participates in histone modification, apoptosis and authophagy, protein stability, RSF complex binding to the centromer, and signal transduction Activin A signaling regulation. HIST1H2BB is known to interact with genes EP300, ERCC6, DYRK1A, NFKB2, and TNFRSF1A. It has been researched regarding its role to lupus, immunodeficiency, cockayne syndrome, and hemochromatosis.

Alternate Names

H2B histone family, member F, H2B/fHistone H2B.f, H2BFFH2B.1, histone 1, H2bb, histone cluster 1, H2bb, histone H2B type 1-B, Histone H2B.1, MGC119804

Gene Symbol

H2BC3

Additional Histone H2B type 1B Products

Product Documents for Histone H2B type 1B [ac Lys20] Antibody (RM235)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H2B type 1B [ac Lys20] Antibody (RM235)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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