Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26027
Recombinant Monoclonal Antibody
![Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26027_rabbit-histone-h2b-type-1b-mab-rm235-azide-and-bsa-free-2462024132616.png "Western Blot: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP3-26027] -")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Vertebrate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM235
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
An acetyl-peptide corresponding to Histone H2B type 1B [ac Lys20]
Specificity
This antibody reacts to Histone H2B type 1B acetylated at Lysine 20. No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free
Western Blot: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP3-26027] -
Western Blot: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP3-26027] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using Clone RM235 at 0.5 ug/mL.![Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26027_rabbit-histone-h2b-type-1b-mab-rm235-azide-and-bsa-free-22620242311089.png "Multiplex Immunoassay: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP3-26027] -")
Multiplex Immunoassay: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP3-26027] -
Multiplex Immunoassay: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP3-26027] - RM235 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.![Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26027_rabbit-histone-h2b-type-1b-mab-rm235-azide-and-bsa-free-24620241245916.png "Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP3-26027] -")
Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP3-26027] -
Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP3-26027] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Clone RM235 (red). Actin filaments have been labeled with fluorescein phalloidin (green).Applications for Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.2 ug/mL-1 ug/mL
Immunocytochemistry/ Immunofluorescence
1 ug/ml - 2 ug/ml
Multiplex Immunoassay
0.1 ug/ml - 0.5 ug/ml
Western Blot
0.5 ug/ml - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2B type 1B
Alternate Names
H2B histone family, member F, H2B/fHistone H2B.f, H2BFFH2B.1, histone 1, H2bb, histone cluster 1, H2bb, histone H2B type 1-B, Histone H2B.1, MGC119804
Gene Symbol
H2BC3
Additional Histone H2B type 1B Products
Product Documents for Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free
Product Specific Notices for Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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