Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18840

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-18840
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Key Product Details

Species Reactivity

Human, Vertebrate

Applications

Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM322

Concentration

1 mg/ml

View all Histone H3 Antibodies »

Product Specifications

Immunogen

A dimethyl-peptide corresponding to Histone H3 [Lys9] and an acetyl-peptide corresponding to Histone H3.3B [Lys14] (Uniprot # P84243)

Modification

Dimethyl Lys9, ac Lys14

Specificity

This antibody reacts to human Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322)

Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322)

Western Blot: Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322) [NBP3-18840] -

Western Blot: Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322) [NBP3-18840] - Western Blot of HeLa cells (1) and recombinant histone H3.3 (2) using RM322 at 0.5 ug/mL.
Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322)

Multiplex Immunoassay: Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322) [NBP3-18840] -

Multiplex Immunoassay: Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322) [NBP3-18840] - RM322 specifically reacts to Histone H3 only when modified at both dimethyl-lysine 9 and acetyl-lysine 14 (K9me2/K14ac). No cross reactivity with non-modified Lysine 9/14, methylated Lysine 9 (K9me1,k9me2, k9me3) ONLY, acetylated Lysine 9/14 ONLY, or methylated Lysine 14 (K14me2) ONLY, in Histone H3.

Applications for Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322)

Application
Recommended Usage

Multiplex Immunoassay

0.01 - 1 ug/mL

Western Blot

0.1 - 1 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Histone H3

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

Alternate Names

H3F3A

Gene Symbol

H3C14

Additional Histone H3 Products

Product Documents for Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H3 [Dimethyl Lys9, ac Lys14] Antibody (RM322)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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