Histone H3 [Trimethyl Lys9] Antibody (RM389)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18839
Recombinant Monoclonal Antibody
![Histone H3 [Trimethyl Lys9] Antibody (RM389)](https://resources.bio-techne.com/images/products/nbp3-18839_rabbit-histone-histone-h3-mab-rm389-161120231301821.png "Western Blot: Histone H3 [Trimethyl Lys9] Antibody (RM389)[NBP3-18839]")
Key Product Details
Species Reactivity
Human, Vertebrate
Applications
ELISA, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM389
Concentration
1 mg/ml
Product Specifications
Immunogen
A trimethyl-peptide corresponding to Histone H3 [Lys9]. (Uniprot # P84243)
Modification
Trimethyl Lys9
Specificity
This antibody reacts to Histone H3 trimethylated at Lysine 9. No cross reactivity with monomethylated Lysine 9, dimethylated Lysine 9, or other methylations in Histone H3.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H3 [Trimethyl Lys9] Antibody (RM389)
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (RM389)[NBP3-18839]
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (RM389)[NBP3-18839]Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM389 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 9 (K9me3) in HeLa cells.![Histone H3 [Trimethyl Lys9] Antibody (RM389)](https://resources.bio-techne.com/images/products/nbp3-18839_rabbit-histone-histone-h3-mab-rm389-17720241044648.png "ELISA: Histone H3 [Trimethyl Lys9] Antibody (RM389) [NBP3-18839] -")
ELISA: Histone H3 [Trimethyl Lys9] Antibody (RM389) [NBP3-18839] -
ELISA: Histone H3 [Trimethyl Lys9] Antibody (RM389) [NBP3-18839] - ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with Histone H3 K4, 9, 27, 36 and H4 K20 Mono-, Di-, and Tri-Methylation), using Clone RM389 (0.5 ug/mL).Applications for Histone H3 [Trimethyl Lys9] Antibody (RM389)
Application
Recommended Usage
ELISA
1 - 10 ug/mL
Multiplex Immunoassay
0.5 - 2 ug/mL
Western Blot
0.5 - 2 ug/mL
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Histone H3
Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).
References
1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030
2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593
3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2
4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8
Alternate Names
H3F3A
Gene Symbol
H3C14
Additional Histone H3 Products
Product Documents for Histone H3 [Trimethyl Lys9] Antibody (RM389)
Product Specific Notices for Histone H3 [Trimethyl Lys9] Antibody (RM389)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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