Histone H3 [Trimethyl Lys79] Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB21-1383
![Western Blot: Histone H3 [Trimethyl Lys79] AntibodyBSA Free [NB21-1383]](https://resources.bio-techne.com/images/products/Histone-H3-[Trimethyl-Lys79]-Antibody-Western-Blot-NB21-1383-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys79] AntibodyBSA Free [NB21-1383]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, C. elegans
Predicted:
Chicken (100%), Drosophila (100%), Plant (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Block/Neutralize, Dot Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.6 mg/ml
Product Specifications
Immunogen
This Histone H3 [Trimethyl Lys79] antibody was raised against synthetic trimethylated peptide surrounding Lysine 79 of human Histone H3 [Swiss Prot Q71DI3].
Reactivity Notes
Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
Modification
Trimethyl Lys79
Localization
Nucleus. Chromosome.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histone H3 [Trimethyl Lys79] Antibody - BSA Free
Western Blot: Histone H3 [Trimethyl Lys79] AntibodyBSA Free [NB21-1383]
Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 in (1) HeLa Histone prep (2) 3T3 Histone prep, and (3) C.elegans embryo lysate. Observed molecular weight is ~16 kDa.
Dot Blot: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Analysis of H3 K79me3 using the peptides listed above in 100, 10, and 1 picomoles of peptide.
Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys79] Antibody [NB21-1383] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1383 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Applications for Histone H3 [Trimethyl Lys79] Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
2 ug/million cells
Dot Blot
1 ug/ml
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1 ug/ml
Application Notes
This Histone H3 K79me3 antibody is useful for ChIP, Western Blot, Immunocytochemistry/Immunofluorescence, and Dot Blot. In Western Blot a band is seen ~15 kDa in C. elegans embryo nuclear extract, HeLa histone prep, and NIH 3T3 histone prep lysates. In ICC/IF, nuclear staining was observed in HeLa cells. In ChIP, increased amplification of actively transcribed loci was seen in HeLa chromatin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
0.6 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H3
Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).
References
1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030
2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593
3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2
4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8
Alternate Names
H3F3A, H3K79Me3, H3pS28K27Me3
Gene Symbol
H3C14
UniProt
Additional Histone H3 Products
Product Documents for Histone H3 [Trimethyl Lys79] Antibody - BSA Free
Product Specific Notices for Histone H3 [Trimethyl Lys79] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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