Histone H3 [Methyl Lys27] Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44208
Key Product Details
Species Reactivity
Human
Applications
Dot Blot, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Histone H3 [Methyl Lys27] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27 me3 site of human Histone H3. (Uniprot: Q71DI3)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [methyl Lys27] from other sources has not been determined.
Modification
Methyl Lys27
Specificity
This antibody recognizes the Me1, Me2, Me3, and acetylated form of Lys27 for human Histone H3.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Histone H3 K27 Methyl Antibody was purified from monospecific antiserum by protein A chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Histone H3 [Methyl Lys27] Antibody - BSA Free
Dot Blot: Histone H3 [Methyl Lys27] Antibody [NBP2-44208] - Histone H3 [Methyl Lys 27] Antibody [NBP2-44208] - Lane 1: Unmodified. Lane 2: me1. Lane 3: me2. Lane 4: me3. Lane 5: ac. Load: 1, 10, and 100 picomoles of peptide.
![Histone H3 [Methyl Lys27] Antibody](https://resources.bio-techne.com/images/products/nbp2-44208_rabbit-polyclonal-histone-h3-methyl-lys27-antibody-23520231753186.jpg "Histone H3 [Methyl Lys27] Antibody")
Histone H3 [Methyl Lys27] Antibody
Dot Blot of Rabbit Histone H3 [Methyl Lys27] Antibody. Lane 1: Unmodified. Lane 2: me1. Lane 3: me2. Lane 4: me3. Lane 5: ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [1-3 methyl, Lys27, ac] antibody at 0.5ug/ml for 45 min at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.Applications for Histone H3 [Methyl Lys27] Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:1000
Application Notes
This product has been tested by dot blot and is suitable for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Histone H3
Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).
References
1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030
2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593
3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2
4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8
Additional Histone H3 Products
Product Documents for Histone H3 [Methyl Lys27] Antibody - BSA Free
Product Specific Notices for Histone H3 [Methyl Lys27] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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