Histone H3 [ac Lys27] Antibody - BSA Free

Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-54615] - Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western Blot: Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.

Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys27] Antibody [NBP2-54615]

Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody [NBP2-54615] - HeLa cells were stained with the antibody against H3K27ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K27ac antibody (top) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown at the bottom.

Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody [NBP2-54615] - ChIP assays were performed using human K562 cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration consisting of 0.2, 0.5, 1 and 2 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as positive controls, and for the coding regions of the inactive MB and MYT1 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis)