Histone H3.1 [Dimethyl Lys9] Antibody (1A10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26621
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1A10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthesized peptide [UniProt P68431]
Modification
Dimethyl Lys 9
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H3.1 [Dimethyl Lys9] Antibody (1A10)
![Histone H3.1 [Dimethyl Lys9] Antibody (1A10)](https://resources.bio-techne.com/images/products/nbp3-26621_rabbit-histone-h3-1-dimethyl-lys-9-mab-1a10-282202411485811.jpg "Western Blot: Histone H3.1 [Dimethyl Lys9] Antibody (1A10) [NBP3-26621] -")
Western Blot: Histone H3.1 [Dimethyl Lys9] Antibody (1A10) [NBP3-26621] -
Western Blot: Histone H3.1 [Dimethyl Lys9] Antibody (1A10) [NBP3-26621] - Positive Western Blot detected in Mouse liver tissue.All lanes: Histone H3.1 [Dimethyl Lys9] Antibody at 0.9ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 15 KDa
Observed band size: 15 KDa
![Histone H3.1 [Dimethyl Lys9] Antibody (1A10)](https://resources.bio-techne.com/images/products/nbp3-26621_rabbit-histone-h3-1-dimethyl-lys-9-mab-1a10-28220241323638.jpg "Immunocytochemistry/Immunofluorescence: Histone H3.1 [Dimethyl Lys9] Antibody (1A10) [NBP3-26621] -")
Immunocytochemistry/Immunofluorescence: Histone H3.1 [Dimethyl Lys9] Antibody (1A10) [NBP3-26621] -
Immunocytochemistry/Immunofluorescence: Histone H3.1 [Dimethyl Lys9] Antibody (1A10) [NBP3-26621] - Staining of Hela cells with Histone H3.1 [Dimethyl Lys9] Antibody (1A10) at 1:56, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).Applications for Histone H3.1 [Dimethyl Lys9] Antibody (1A10)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:30-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Histone H3.1
Long Name
Histone Cluster 1 H3 Family Member A
Alternate Names
HIST1H3A
Gene Symbol
HIST1H3E
Additional Histone H3.1 Products
Product Documents for Histone H3.1 [Dimethyl Lys9] Antibody (1A10)
Product Specific Notices for Histone H3.1 [Dimethyl Lys9] Antibody (1A10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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