Histone H3.1 [Monomethyl Lys18] Antibody (2B5)

Western Blot: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] -

Western Blot: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Positive Western Blot detected in MCF-7 whole cell lysate. Hela whole cell lysate. HepG2 whole cell lysate. SH-SY5Y whole cell lysate, Rat brain tissue.
All lanes: Histone H3.1 [Monomethyl Lys18] Antibody at 0.7ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 15 KDa
Observed band size: 15 KDa

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] -

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Analysis of Histone H3.1 [Monomethyl Lys18] Antibody (2B5) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] -

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Staining of Hela cells with Histone H3.1 [Monomethyl Lys18] Antibody (2B5) at 1:43, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).