Histone H3.1 [ac Lys56] Antibody (3H2)

Western Blot: Histone H3.1 [ac Lys56] Antibody (3H2) [NBP3-26534] -

Western Blot: Histone H3.1 [ac Lys56] Antibody (3H2) [NBP3-26534] - Positive Western Blot detected in: Hela whole cell lysate treated by 15mM sodium butyrate for 30min.
All lanes: Histone H3.1 [ac Lys56] Antibody at 0.7ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 15 KDa
Observed band size: 15 KDa

Immunocytochemistry/Immunofluorescence: Histone H3.1 [ac Lys56] Antibody (3H2) [NBP3-26534] -

Immunocytochemistry/Immunofluorescence: Histone H3.1 [ac Lys56] Antibody (3H2) [NBP3-26534] - Analysis of Histone H3.1 [ac Lys56] Antibody (3H2) diluted at 1:100 and staining in HepG2 cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/Immunofluorescence: Histone H3.1 [ac Lys56] Antibody (3H2) [NBP3-26534] -

Immunocytochemistry/Immunofluorescence: Histone H3.1 [ac Lys56] Antibody (3H2) [NBP3-26534] - Staining of Hela cells(treated by 15mM sodium butyrate for 30min) with Histone H3.1 [ac Lys56] Antibody (3H2) at 1:43, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).