Skip to main content

Histone H3.1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75524

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-75524

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within Human Histone H3.1 aa 1-50 / 136. (SwissProt: P68431 Human; SwissProt: P68433 Mouse)

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H3.1 Antibody

Western Blot: Histone H3.1 Antibody [NBP2-75524]

Western Blot: Histone H3.1 Antibody [NBP2-75524]

Western Blot: Histone H3.1 Antibody [NBP2-75524] - Analysis of Histone H3.1 on F9 cell lysate using anti-Histone H3.1 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: Histone H3.1 Antibody [NBP2-75524]

Immunocytochemistry/ Immunofluorescence: Histone H3.1 Antibody [NBP2-75524]

Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Histone H3.1 Antibody [NBP2-75524]

Immunohistochemistry-Paraffin: Histone H3.1 Antibody [NBP2-75524]

Immunohistochemistry-Paraffin: Histone H3.1 Antibody [NBP2-75524] - Analysis of paraffin-embedded human liver tissue using anti-Histone H3.1 antibody. Counter stained with hematoxylin.

Applications for Histone H3.1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H3.1

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of Histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser 10 and Ser 28, both of which are extensively phosphorylated in DNA-bound forms of Histone H3 and in nucleosomal Histone H3. The nucleosome structure of Histone H3 promotes N-terminal phosphorylation in vitro. Phosphorylation of Histone H3 at Ser 10 is an essenti

Long Name

Histone Cluster 1 H3 Family Member A

Alternate Names

HIST1H3A

Gene Symbol

HIST1H3E

Additional Histone H3.1 Products

Product Documents for Histone H3.1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone H3.1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...