Histone H4 [ac Lys12] Antibody (RM202) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26016CL1

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP2-61554

Alexa Fluor 350

NBP3-26016AF350

Alexa Fluor 405

NBP3-26016AF405

Alexa Fluor 488

NBP3-26016AF488

Alexa Fluor 532

NBP3-26016AF532

Alexa Fluor 594

NBP3-26016AF594

Alexa Fluor 647

NBP3-26016AF647

Alexa Fluor 700

NBP3-26016AF700

Alexa Fluor 750

NBP3-26016AF750

Allophycocyanin

NBP3-26016APC

Biotin

NBP3-26016B

DyLight 350

NBP3-26016UV

DyLight 405

NBP3-26016V

DyLight 488

NBP3-26016G

DyLight 550

NBP3-26016R

DyLight 594

NBP3-26016DL594

DyLight 650

NBP3-26016C

DyLight 680

NBP3-26016FR

DyLight 755

NBP3-26016IR

FITC

NBP3-26016F

HRP

NBP3-26016H

Janelia Fluor 525

NBP3-26016JF525

Janelia Fluor 549

NBP3-26016JF549

Janelia Fluor 646

NBP3-26016JF646

Janelia Fluor 669

NBP3-26016JF669

mFluor Violet 450 SE

NBP3-26016MFV450

mFluor Violet 500 SE

NBP3-26016MFV500

mFluor Violet 610 SE

NBP3-26016MFV610

PE

NBP3-26016PE

PerCP

NBP3-26016PCP

Forumulation

Catalog #

Azide and BSA Free

NBP3-26016

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Vertebrate

Applications

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

CoraFluor 1

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM202

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H4 Antibodies »

Product Specifications

Immunogen

An acetyl-peptide corresponding to Histone H4 [ac Lys12]

Modification

ac Lys12

Specificity

This antibody reacts to Histone H4 acetylated at Lysine 12. No cross reactivity with other acetylated Lysines in Histone H4.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Histone H4 [ac Lys12] Antibody (RM202) [CoraFluor™ 1]

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Multiplex Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Histone H4

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents for Histone H4 [ac Lys12] Antibody (RM202) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Histone H4 [ac Lys12] Antibody (RM202) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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