Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26013
Recombinant Monoclonal Antibody
![Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26013_rabbit-histone-h4-mab-rm205-azide-and-bsa-free-226202422321211.png "Western Blot: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] -")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Vertebrate
Applications
Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM205
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free
Immunogen
An acetyl-peptide corresponding to the Histone H4 [ac Lys20]
Specificity
This antibody reacts to Histone H4 acetylated at Lysine 20. No cross reactivity with other acetylated Lysines in Histone H4.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free
Western Blot: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] -
Western Blot: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4, using Clone RM205 at 1 ug/mL, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells.![Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26013_rabbit-histone-h4-mab-rm205-azide-and-bsa-free-2462024132646.png "Multiplex Immunoassay: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] -")
Multiplex Immunoassay: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] -
Multiplex Immunoassay: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] - RM205 specifically reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.![Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26013_rabbit-histone-h4-mab-rm205-azide-and-bsa-free-226202422174414.png "Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] -")
Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] -
Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free [NBP3-26013] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Clone RM205 (red). Actin filaments have been labeled with fluorescein phalloidin (green).Applications for Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1ug-5 ug
ELISA
0.2 ug/ml - 1 ug/ml
Immunocytochemistry/ Immunofluorescence
1 ug/ml - 2 ug/ml
Multiplex Immunoassay
0.1 ug/ml - 1 ug/ml
Western Blot
1 ug/ml - 2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H4
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4
Gene Symbol
H4C16
Additional Histone H4 Products
Product Documents for Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free
Product Specific Notices for Histone H4 [ac Lys20] Antibody (RM205) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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