Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26024
Recombinant Monoclonal Antibody
![Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26024_rabbit-histone-h4-mab-rm208-azide-and-bsa-free-226202422321225.png "Multiplex Immunoassay: Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free [NBP3-26024] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Vertebrate
Applications
ELISA, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM208
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A trimethyl-peptide corresponding to Histone H4 [Methyl Lys20]
Specificity
This antibody reacts to Histone H4 trimethylated at Lysine 20. It may slightly cross react to dimethylated Lysine 20 at a higher concentration. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20, or other methylations in Histone H4.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free
Multiplex Immunoassay: Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free [NBP3-26024] -
Multiplex Immunoassay: Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free [NBP3-26024] - RM208 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) when used at higher concentrations. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.![Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26024_rabbit-histone-h4-mab-rm208-azide-and-bsa-free-226202422321242.png "Western Blot: Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free [NBP3-26024] -")
Western Blot: Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free [NBP3-26024] -
Western Blot: Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free [NBP3-26024] - Western Blot of acid extracts of HeLa cells (1) and recombinant Histone H4 (2), using RM208 at 1 ug/mL, showed a band of Histone H4 trimethylated at Lysine 20 in HeLa cells.Applications for Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.5 ug/mL - 1 ug/mL
Multiplex Immunoassay
0.1 ug/ml - 0.5 ug/ml
Western Blot
1 ug/ml - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H4
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4
Gene Symbol
H4C16
Additional Histone H4 Products
Product Documents for Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free
Product Specific Notices for Histone H4 [Trimethyl Lys20] Antibody (RM208) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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