Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26036
Recombinant Monoclonal Antibody
![Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26036_rabbit-histone-h4-mab-rm457-azide-and-bsa-free-226202422321226.png "Multiplex Immunoassay: Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free [NBP3-26036] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Vertebrate
Applications
ELISA, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM457
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A monomethyl-peptide corresponding to Histone H4 [Lys5] (Uniprot # P62805)
Specificity
This antibody reacts to Histone H4 monomethylated at Lysine 5. No cross reactivity with non-modified Lysine 5, or other monomethylations in histone H4.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free
Multiplex Immunoassay: Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free [NBP3-26036] -
Multiplex Immunoassay: Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free [NBP3-26036] - RM457 specifically reacts to Histone H4 monomethylated at Lysine 5 (K5me1). No cross reactivity with non-modified Lysine 5, or other monomethylations in histone H4.![Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26036_rabbit-histone-h4-mab-rm457-azide-and-bsa-free-2462024124590.png "Western Blot: Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free [NBP3-26036] -")
Western Blot: Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free [NBP3-26036] -
Western Blot: Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free [NBP3-26036] - Western Blot of acid extracts of HeLa cells (2) and recombinant histone H4 (1), using RM457 at 0.2 ug/mL, showed a band of histone H4 monomethylated at Lysine 5 in HeLa cells.Applications for Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.5 ug/mL - 1 ug/mL
Multiplex Immunoassay
0.2 ug/mL - 5 ug/mL
Western Blot
0.1 ug/mL - 2 ug/mL
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H4
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4
Gene Symbol
H4C16
Additional Histone H4 Products
Product Documents for Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free
Product Specific Notices for Histone H4 [Monomethyl Lys5] Antibody (RM457) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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