Histone H4 [Monomethyl Arg3] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB21-2011
![Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]](https://resources.bio-techne.com/images/products/Histone-H4-[Monomethyl-Arg3]-Antibody-Western-Blot-NB21-2011-img0002.jpg "Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Mammal
Cited:
Human, Mouse
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.43 mg/ml
Product Specifications
Immunogen
Synthetic monomethylated peptide surrounding Arginine 3 of human Histone H4 [Swiss Prot P62805].
Modification
Monomethyl Arg3
Localization
Nucleus. Chromosome.
Marker
H4R3me1
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H4 [Monomethyl Arg3] Antibody
Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]
Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Analysis of Histone H4 R3me1 in NIH/3T3 histone preps.![Immunocytochemistry/ Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]](https://resources.bio-techne.com/images/products/Histone-H4-[Monomethyl-Arg3]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2011-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]")
Immunocytochemistry/ Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]
Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Histone H4 R3me1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]
Applications for Histone H4 [Monomethyl Arg3] Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
2-5 ug per million cells
Dot Blot
1:1000
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:1000
Application Notes
In Western blot a band is seen ~13 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.43 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H4
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4, H4R3Me1
Gene Symbol
H4-16
UniProt
Additional Histone H4 Products
Product Documents for Histone H4 [Monomethyl Arg3] Antibody
Product Specific Notices for Histone H4 [Monomethyl Arg3] Antibody
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc. *Chromatin immunoprecipitation was performed on this product with a standard set of primers that amplify active gene loci (GAPDH, RPL30), inactive loci (MYOD1, AFM), and heterochromatic loci (alphaSat, SAT2). The resulting data indicated there was little to no amplification of these sites, which corresponds with the current literature. Until a more specific locus can be established and validated, we give this product validity for ChIP and it is backed by the full Novus guarantee.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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