Histone H4 [ac Lys8] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB21-2044

Novus Biologicals, part of Bio-Techne
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NB21-2044
NB21-2044SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, C. elegans

Applications

Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.85 mg/ml

View all Histone H4 Antibodies »

Product Summary for Histone H4 [ac Lys8] Antibody - BSA Free

Immunogen

Synthetic acetylated peptide surrounding lysine 8 of human histone H4 [Swiss Prot P62805].

Modification

ac Lys8

Localization

Nucleus.

Marker

H4K8ac

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H4 [ac Lys8] Antibody - BSA Free

Western Blot: Histone H4 [ac Lys8] AntibodyBSA Free [NB21-2044]

Western Blot: Histone H4 [ac Lys8] AntibodyBSA Free [NB21-2044]

Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.
Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys8] Antibody - BSA Free [NB21-2044]

Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys8] Antibody - BSA Free [NB21-2044]

Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NB21-2044] - H4 K8ac antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NB21-2044] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-2044 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Applications for Histone H4 [ac Lys8] Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

2 ug/million cells

Dot Blot

1:1000

Immunocytochemistry/ Immunofluorescence

1:1000-1:2000

Western Blot

0.02 ug/ml
Application Notes
This H4K8ac antibody is useful for ChIP, Dot blot, Immunocytochemistry/Immunofluorescence and Western blot. In WB a band is detected ~11 kDa in HeLa, NIH-3T3, and C. elegans embyro preps. In ICC/IF, nuclear staining was observed in HeLa cells. ChIP experiments detected increased amplification of actively transcribed loci in chromatin prepared from HeLa cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.85 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H4

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys5 (H4 Lys5Ac) has been linked to transcriptional activation and DNA repair. Newly assembled histones are typically acetylated on H4 at lysine 5 and 12. The enzyme histone acetyltransferase 1 (HAT1) is the primary modulator, and these marks are necessary for complete chromatin assembly.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4, H4K8ac

Entrez Gene IDs

121504 (Human); 320332 (Mouse)

Gene Symbol

H4-16

UniProt

P62805 (Human), P62806 (Mouse)

Additional Histone H4 Products

Product Documents for Histone H4 [ac Lys8] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H4 [ac Lys8] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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