Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin (Negative), Immunoprecipitation

Cited:

ELISA, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Magnetic Cell Separation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # W6/32

Concentration

1 mg/ml

View all HLA ABC Antibodies »

Product Specifications

Immunogen

Purified human tonsil lymphocyte membranes

Reactivity Notes

Mouse reactivity reported in scientific literature (Parasar P et al).

Specificity

NB100-64775 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for HLA ABC Antibody (W6/32)

Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis of Mouse colon cancer tissue using HLA ABC antibody (W6/32). Primary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution. Image from verified customer review.
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC.
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Applications for HLA ABC Antibody (W6/32)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
Antibody does not work in Immunohistochemistry-Paraffin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-64775 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HLA ABC

HLA CLass I is involved in the presentation of foreign antigens to the immune system. HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

Alternate Names

A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80

Gene Symbol

HLA-A

Additional HLA ABC Products

Product Documents for HLA ABC Antibody (W6/32)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HLA ABC Antibody (W6/32)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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