HLA Class I Antibody (MEM-123) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-505

Novus Biologicals, part of Bio-Techne
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NB500-505
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Key Product Details

Species Reactivity

Human, Bovine, Primate

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # MEM-123

Format

BSA Free

Concentration

1.0 mg/ml

View all HLA Class I Antibodies »

Product Specifications

Immunogen

COS-7 African green monkey kidney cells

Specificity

This antibody (clone MEM-123) reacts with all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. This antibody completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks This antibody MEM-G/9.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for HLA Class I Antibody (MEM-123) - BSA Free

Flow Cytometry: HLA Class I Antibody (MEM-123) - BSA Free [NB500-505]

Flow Cytometry: HLA Class I Antibody (MEM-123) - BSA Free [NB500-505]

Flow Cytometry: HLA Class I Antibody (MEM-123) [NB500-505] - Surface staining of human peripheral blood with anti-HLA-class I (MEM-123) purified, GAM-APC.
Flow Cytometry: HLA Class I Antibody (MEM-123) - BSA Free [NB500-505]

Flow Cytometry: HLA Class I Antibody (MEM-123) - BSA Free [NB500-505]

Flow Cytometry: HLA Class I Antibody (MEM-123) [NB500-505] - A surface stain was performed on human peripheral blood lymphocytes with HLA Class I (MEM-123) antibody NB500-505 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Applications for HLA Class I Antibody (MEM-123) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Flow Cytometry

4 ug/ml

Immunoprecipitation

1:50
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Tris buffered saline (TBS), pH 8.0

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HLA Class I

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Long Name

Class I Human Leukocyte Antigen

Alternate Names

HLA-A, HLA-ABC

Entrez Gene IDs

3107 (Human); 3106 (Human)

Gene Symbol

HLA-C

Additional HLA Class I Products

Product Documents for HLA Class I Antibody (MEM-123) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA Class I Antibody (MEM-123) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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