HLA Class I Antibody (MEM-147) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-411
![Western Blot: HLA Class I Antibody (MEM-147)BSA Free [NB500-411]](https://resources.bio-techne.com/images/products/HLA-Class-I-Antibody-MEM-147-Western-Blot-NB500-411-img0002.jpg "Western Blot: HLA Class I Antibody (MEM-147)BSA Free [NB500-411]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-147
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
PHA-activated peripheral blood lymphocytes
Specificity
This antibody (clone MEM-147) reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HLA Class I Antibody (MEM-147) - BSA Free
Western Blot: HLA Class I Antibody (MEM-147)BSA Free [NB500-411]
Western Blot: HLA Class I Antibody (MEM-147) [NB500-411] - Human Ramos B cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb MEM-147 and goat anti-mouse IgG (H+L)-HRP conjugate.Applications for HLA Class I Antibody (MEM-147) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-5 ug/ml
Immunoprecipitation
1:50
Western Blot
1:1000
Application Notes
Western Blot: Non-reducing conditions. This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HLA Class I
Long Name
Class I Human Leukocyte Antigen
Alternate Names
HLA-A, HLA-ABC
Gene Symbol
HLA-C
Additional HLA Class I Products
Product Documents for HLA Class I Antibody (MEM-147) - BSA Free
Product Specific Notices for HLA Class I Antibody (MEM-147) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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