HLA Class I Antibody (MEM-147) [DyLight 680]
Novus Biologicals, part of Bio-Techne | Catalog # NB500-411FR
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot
Label
DyLight 680 (Excitation = 692 nm, Emission = 712 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-147
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PHA-activated peripheral blood lymphocytes
Specificity
This antibody (clone MEM-147) reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for HLA Class I Antibody (MEM-147) [DyLight 680]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: HLA Class I
Long Name
Class I Human Leukocyte Antigen
Alternate Names
HLA-A, HLA-ABC
Gene Symbol
HLA-C
Additional HLA Class I Products
Product Documents for HLA Class I Antibody (MEM-147) [DyLight 680]
Product Specific Notices for HLA Class I Antibody (MEM-147) [DyLight 680]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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