HLA DMA Antibody (5C9NB) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44300

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-44300
NBP2-44300SS
Print Quote
Bulk Order
Forumulation
Catalog #
Azide Free
NBP2-44301
View Product
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5C9NB

Format

BSA Free

Concentration

1.0 mg/ml

View all HLA DMA Antibodies »

Product Specifications

Immunogen

Partial recombinant human HLA DMA protein (between amino acids 10-300) [Entrez NP_006111]

Localization

Membranes of Late Endosomes / Lysosomes

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HLA DMA Antibody (5C9NB) - BSA Free

Western Blot: HLA DMA Antibody (5C9NB)BSA Free [NBP2-44300]

Western Blot: HLA DMA Antibody (5C9NB)BSA Free [NBP2-44300]

Western Blot: HLA DMA Antibody (5C9NB) [NBP2-44300] - Western blot analysis of HLA DMA (5C9NB) in A. partial recombinant human HLA DMA protein B. Ramos lysate and C. Raji lysate.
Immunocytochemistry/ Immunofluorescence: HLA DMA Antibody (5C9NB) - BSA Free [NBP2-44300]

Immunocytochemistry/ Immunofluorescence: HLA DMA Antibody (5C9NB) - BSA Free [NBP2-44300]

Immunocytochemistry/Immunofluorescence: HLA DMA Antibody (5C9NB) [NBP2-44300] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with MHC Class II (5C9NB) at a 1:40 dilution for 1 hour at room temperature and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red). Nuclei were detected using DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: HLA DMA Antibody (5C9NB) - BSA Free [NBP2-44300]

Immunohistochemistry: HLA DMA Antibody (5C9NB) - BSA Free [NBP2-44300]

Immunohistochemistry: HLA DMA Antibody (5C9NB) [NBP2-44300] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone 5C9NB). The antibody generated a diffused to punctate staining in cellular membranes and cytoplasm, especially in the perinuclear region representing late endosomal localization of major histocompatibility complex, class II, DM alpha/HLA DMA protein.
View 1 more images

Applications for HLA DMA Antibody (5C9NB) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

2.5 ug / million cells

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA DMA

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.

Alternate Names

class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM

Gene Symbol

HLA-DMA

Additional HLA DMA Products

Product Documents for HLA DMA Antibody (5C9NB) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HLA DMA Antibody (5C9NB) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.