HLA DMA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85325

Novus Biologicals, part of Bio-Techne
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NBP1-85325
NBP1-85325-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HLA DMA Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PTPMWPDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFFFDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFCEWMIQQIGPKLDGKIPVSRGFPIAEV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLA DMA Antibody

Western Blot: HLA DMA Antibody [NBP1-85325]

Western Blot: HLA DMA Antibody [NBP1-85325]

Western Blot: HLA DMA Antibody [NBP1-85325] - Analysis in control (vector only transfected HEK293T lysate) and HLA-DMA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325]

Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325]

Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325] - Staining in human tonsil and cerebral cortex tissues using anti-HLA-DMA antibody. Corresponding HLA-DMA RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325]

Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325]

Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325] - Staining of human cerebral cortex shows low expression as expected.
View 1 more images

Applications for HLA DMA Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA DMA

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.

Alternate Names

class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM

Gene Symbol

HLA-DMA

Additional HLA DMA Products

Product Documents for HLA DMA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA DMA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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