HLA DPA Antibody (1E3)

Novus Biologicals, part of Bio-Techne | Catalog # H00003113-M03

Novus Biologicals, part of Bio-Techne
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H00003113-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HLA DPA Antibodies »

Product Specifications

Immunogen

HLA-DPA1 (AAH09956, 1 a.a. ~ 260 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRPEDRMFHIRAVILRALSLAFLLSLRGAGAIKADHVSTYAAFVQTHRPTGEFMFEFDEDEQFYVDLDKKETVWHLEEFGRAFSFEAQGGLANIAILNNNLNTLIQRSNHTQAANDPPEVTVFPKEPVELGQPNTLICHIDRFFPPVLNVTWLCNGEPVTEGVAESLFLPRTDYSFHKFHYLTFVPSAEDVYDCRVEHWGLDQPLLKHWEAQEPIQMPETTETVLCALGLVLGLVGIIVGTVLIIKSLRSGHDPRAQGPL

Specificity

HLA-DPA1 - major histocompatibility complex, class II, DP alpha 1 (1E3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HLA DPA Antibody (1E3)

Western Blot: HLA DPA Antibody (1E3) [H00003113-M03]

Western Blot: HLA DPA Antibody (1E3) [H00003113-M03]

Western Blot: HLA DPA Antibody (1E3) [H00003113-M03] - Analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3. Lane 1: HLA-DPA1 transfected lysatE (29.3 KDa). Lane 2: Non-transfected lysate.
ELISA: HLA DPA Antibody (1E3) [H00003113-M03]

ELISA: HLA DPA Antibody (1E3) [H00003113-M03]

ELISA: HLA DPA Antibody (1E3) [H00003113-M03] - Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3ng/ml as a capture antibody.

Applications for HLA DPA Antibody (1E3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLA DPA

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq]

Alternate Names

DP alpha 1 chain, HLA class II histocompatibility antigen, DP alpha chain, HLADP, HLA-SB alpha chain, major histocompatibility complex, class II, DP alpha 1, MHC class II antigen, MHC class II DP3-alpha, MHC class II DPA1, MHC class II HLA-DPA1 antigen, Primed lymphocyte test-1

Entrez Gene IDs

3113 (Human)

Gene Symbol

HLA-DPA1

UniProt

AAH09956 (Human)

Additional HLA DPA Products

Product Documents for HLA DPA Antibody (1E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA DPA Antibody (1E3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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