HLA DQ/DR/DP Antibody (rHLA-Pan/3475)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07808

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-07808-100ug
NBP3-07808-20ug
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rHLA-Pan/3475

Concentration

0.2 mg/ml

View all HLA DQ/DR/DP Antibodies »

Product Specifications

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cells

Localization

Cell Surface

Specificity

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08644)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HLA DQ/DR/DP Antibody (rHLA-Pan/3475)

Western Blot: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808]

Western Blot: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808]

Western Blot: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Western Blot Analysis of (1) Ramos cell and (2) human spleen tissue lysate using HLA DQ/DR/DP antibody (rHLA-Pan/3475).
Immunocytochemistry/ Immunofluorescence: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808]

Immunocytochemistry/ Immunofluorescence: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808]

Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Immunofluorescence Analysis of Human Raji cells with HLA DQ/DR/DP Mouse Recombinant Monoclonal Antibody (rHLA DQ/DR/DP/3475) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).
Immunohistochemistry-Paraffin: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808]

Immunohistochemistry-Paraffin: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808]

Immunohistochemistry-Paraffin: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DQ/DR/DP Mouse Recombinant Monoclonal Antibody (rHLA DQ/DR/DP/3475).
View 1 more images

Applications for HLA DQ/DR/DP Antibody (rHLA-Pan/3475)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HLA DQ/DR/DP

The major histocompatibility complex (MHC) is a large genomic region or gene family found in most vertebrates containing many genes with important immune system roles. In humans, the MHC spans almost 4 megabases of chromosome 6 and includes more than 200 known genes, of which about half have known immmunological functions. The best known genes in the MHC region are the subset that encodes cell-surface antigen-presenting proteins. In humans, these genes are referred to as human leukocyte antigen (HLA) genes. The most intensely studied HLA genes are: HLA A, HLA B, HLA C, HLA DPA, HLA DPB1, HLA DQA1, HLA DQB1, HLA DRA, and HLA DRB1. In humans, the HLA is divided into three regions: Class I, II, and III. The A, B, and C genes belong to HLA class I while the six D genes belong to class II.

Alternate Names

CD, CELIAC1DQ alpha 1 chain, DC-1 alpha chain, DQ-A1, FLJ27088, FLJ27328, GSE, HLA class II histocompatibility antigen, DQ(W3) alpha chain, HLA-DCA, HLA-DQA, leucocyte antigen DQA1, leukocyte antigen alpha chain, major histocompatibility complex, class II, DQ alpha 1, MGC149527, MHC class II antigen, MHC class II DQA1, MHC class II HLA-D alpha glycoprotein, MHC class II HLA-DQ-alpha-1, MHC class II surface glycoprotein, MHC HLA-DQ alpha

Gene Symbol

HLA-DQA1

UniProt

P01903, P01920 (Human), P04440 (Human), P01909 (Human), P01908 (Human)

Additional HLA DQ/DR/DP Products

Product Documents for HLA DQ/DR/DP Antibody (rHLA-Pan/3475)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA DQ/DR/DP Antibody (rHLA-Pan/3475)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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