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HLA-E Antibody (MEM-E/07) [Janelia Fluor® 585]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-310JF585

Novus Biologicals, part of Bio-Techne
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NB500-310JF585
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Janelia Fluor 585

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-E/07

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for HLA-E Antibody (MEM-E/07) [Janelia Fluor® 585]

Immunogen

Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

Localization

Type I membrane protein.

Specificity

The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for HLA-E Antibody (MEM-E/07) [Janelia Fluor® 585]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HLA-E

Long Name

Major Histocompatibility Complex, Class I, E

Alternate Names

HLA-6.2, HLAE, QA1

Gene Symbol

HLA-E

Additional HLA-E Products

Product Documents for HLA-E Antibody (MEM-E/07) [Janelia Fluor® 585]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HLA-E Antibody (MEM-E/07) [Janelia Fluor® 585]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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