HLA G Antibody (01G) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44926
![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]](https://resources.bio-techne.com/images/products/HLA-G-Antibody-01G-BSA-Free-Flow-Cytometry-NBP1-44926-img0003.jpg "Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]")
![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]](https://resources.bio-techne.com/images/products/HLA-G-Antibody-01G-BSA-Free-Flow-Cytometry-NBP1-44926-img0004.jpg "Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse (Negative), Rat (Negative)
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 01G
Format
BSA Free
Concentration
1 mg/ml
Product Summary for HLA G Antibody (01G) - BSA Free
Immunogen
HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA G Antibody (01G) and human beta2-microglobulin.
Specificity
The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HLA G Antibody (01G) - BSA Free
Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]
Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]](https://resources.bio-techne.com/images/products/HLA-G-Antibody-01G-Flow-Cytometry-NBP1-44926-img0001.jpg "Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]")
Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]
Flow Cytometry: HLA G Antibody (01G) [NBP1-44926] - Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) purified, GAM-APC.![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]](https://resources.bio-techne.com/images/products/HLA-G-Antibody-01G-Flow-Cytometry-NBP1-44926-img0002.jpg "Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]")
Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926]
Flow Cytometry: HLA G Antibody (01G) [NBP1-44926] - Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) FITC.Applications for HLA G Antibody (01G) - BSA Free
Application
Recommended Usage
ELISA
1:2000
Flow Cytometry
2-4 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:200
Immunoprecipitation
1:50
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HLA-G
Discovered in cytotrophoblasts, HLA-G is involved in fetal maternal immune tolerance and some studies have linked its downregulation with severe preeclampsia (3). HLA-G mediated immune suppression works by impeding cell proliferation, differentiation, cytotoxicity, cytokine secretion and chemotaxis; and activation of regulatory cells and MDSCs or M2 type macrophage. HLA-G is constitutively expressed in immune-privileged sites such as pancreatic islets, mesenchymal stem cells (MSCs) and the cornea. Upregulation of HLA-G occurs in pathological states including cancer, allo-transplantations, viral infections, autoimmune and inflammatory diseases (4). In cancer, HLA-G expression is induced by hypoxia and has been correlated with advanced tumor stage, tumor metastasis, and poor therapeutic response and survival. HLA-G is an attractive tumor associated-antigen (TAA) for immunotherapy and is considered a major immune checkpoint (ICP).
References
1. Loustau, M., Anna, F., Drean, R., Lecomte, M., Langlade-Demoyen, P., & Caumartin, J. (2020). HLA-G Neo-Expression on Tumors. Front Immunol, 11:1685. PMID: 32922387
2. Lin A, Yan WH. (2018) Heterogeneity of HLA-G Expression in Cancers: Facing the Challenges. Front Immunol. 9:2164. PMID: 30319626
3. Djurisic S, Hviid TV. (2014) HLA Class Ib Molecules and Immune Cells in Pregnancy and Preeclampsia. Front Immunol. 5:652. PMID: 25566263
4. Lila N, Rouas-Freiss N, Dausset J, Carpentier A, Carosella ED. (2001) Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 98(21):12150-12155. PMID: 11572934
Long Name
Major Histocompatibility Complex, Class I, G
Alternate Names
HLA G, HLA-6.0, HLAG, MHC Class I Antigen G, MHC-G
Gene Symbol
HLA-G
Additional HLA-G Products
Product Documents for HLA G Antibody (01G) - BSA Free
Product Specific Notices for HLA G Antibody (01G) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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