HLRC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91994

Novus Biologicals, part of Bio-Techne
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NBP1-91994-25ul
NBP1-91994
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HLRC1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLRC1 Antibody

Western Blot: HLRC1 Antibody [NBP1-91994]

Western Blot: HLRC1 Antibody [NBP1-91994]

Western Blot: HLRC1 Antibody [NBP1-91994] - Analysis in control (vector only transfected HEK293T lysate) and DOHH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: HLRC1 Antibody [NBP1-91994]

Immunohistochemistry-Paraffin: HLRC1 Antibody [NBP1-91994]

Immunohistochemistry-Paraffin: HLRC1 Antibody [NBP1-91994] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: HLRC1 Antibody [NBP1-91994]

Immunohistochemistry-Paraffin: HLRC1 Antibody [NBP1-91994]

Immunohistochemistry-Paraffin: HLRC1 Antibody [NBP1-91994] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
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Applications for HLRC1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLRC1

Deoxyhypusine hydroxylase (DOHH; EC 1.14.99.29) catalyzes the final step in the formation of the amino acid hypusine in eukaryotic initiation factor-5A (EIF5A; see MIM 600187).[supplied by OMIM]

Alternate Names

Deoxyhypusine dioxygenase, deoxyhypusine hydroxylase, deoxyhypusine hydroxylase/monooxygenase, Deoxyhypusine monooxygenase, EC 1.14.99.29, hDOHH, HEAT-like (PBS lyase) repeat containing 1, HEAT-like repeat-containing protein 1, HLRC1, MGC4293

Gene Symbol

DOHH

Additional HLRC1 Products

Product Documents for HLRC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLRC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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