HLTF Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1041
Key Product Details
Species Reactivity
Validated:
Human, Primate
Cited:
Human, Primate
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Peptide ELISA, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KQAKINEIRTLIDL corresponding to C-Terminus according to NP_003062.2, NP_620636.1.
Reactivity Notes
Primate reactivity reported in scientific literature (PMID: 23415230). Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.
Specificity
NP_003062.2 and NP_620636.2 are varients that represent the same protein.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HLTF Antibody
![Western Blot: HLTF Antibody [NB100-1041]](https://resources.bio-techne.com/images/products/HLTF-Antibody-Western-Blot-NB100-1041-img0002.jpg "Western Blot: HLTF Antibody [NB100-1041]")
Western Blot: HLTF Antibody [NB100-1041]
Western Blot: HLTF Antibody [NB100-1041] - staining (0.5ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.![Flow Cytometry: HLTF Antibody [NB100-1041]](https://resources.bio-techne.com/images/products/HLTF-Antibody-Flow-Cytometry-NB100-1041-img0001.jpg "Flow Cytometry: HLTF Antibody [NB100-1041]")
Flow Cytometry: HLTF Antibody [NB100-1041]
Flow Cytometry: HLTF Antibody [NB100-1041] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for HLTF Antibody
Application
Recommended Usage
Flow Cytometry
10ug/ml
Peptide ELISA
Detection limit 1:16000
Western Blot
0.3-1ug/ml
Application Notes
Western blot: Approx. 125kDa band observed in nuclear lysates of cell line HeLa and in preliminary testing of A431 nuclear cell lysate (calculated MW of 114kDa according to NP_003062.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HLTF
Alternate Names
DNA-binding protein/plasminogen activator inhibitor 1 regulator, EC 3.6.1, EC 3.6.4.-, EC 6.3.2.-, helicase-like transcription factor, HIP116ARING finger protein 80, HIP116matrix associated, actin dependent regulator of chromatin, RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3, subfamily a, member 3, Sucrose nonfermenting protein 2-like 3, ZBU1
Gene Symbol
HLTF
UniProt
Additional HLTF Products
Product Documents for HLTF Antibody
Product Specific Notices for HLTF Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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