HMGA1 Antibody (5J0E6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16379

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16379-100ul
NBP3-16379-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5J0E6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HMGA1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-107 of human HMGA1 (P17096). MSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMGA1 Antibody (5J0E6)

Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379]

Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379]

Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of extracts of Mouse testis, using HMGA1 Rabbit mAb (NBP3-16379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: HMGA1 Antibody (5J0E6) [NBP3-16379]

Immunohistochemistry-Paraffin: HMGA1 Antibody (5J0E6) [NBP3-16379]

Immunohistochemistry-Paraffin: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry of paraffin-embedded human oophoroma using HMGA1 Rabbit mAb (NBP3-16379) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379]

Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379]

Western Blot: HMGA1 Antibody (5J0E6) [NBP3-16379] - Western blot analysis of extracts of various cell lines, using HMGA1 Rabbit mAb (NBP3-16379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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Applications for HMGA1 Antibody (5J0E6)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

3ug antibody for 10ug-15ug of Chromatin

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunohistochemistry

1:2000 - 1:8000

Immunohistochemistry-Paraffin

1:2000 - 1:8000

Western Blot

1:1000 - 1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HMGA1

HMGA1 is expected to recognise isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and isoform b (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1).

Alternate Names

high mobility group AT-hook 1, High mobility group AT-hook protein 1, High mobility group protein A1, high mobility group protein HMG-I/HMG-Y, High mobility group protein R, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, HMGA1A, HMG-I(Y), HMGIYMGC12816, HMG-R, MGC4242, MGC4854, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y

Gene Symbol

HMGA1

Additional HMGA1 Products

Product Documents for HMGA1 Antibody (5J0E6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HMGA1 Antibody (5J0E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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