HMGA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13068

Novus Biologicals, part of Bio-Techne
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NBP3-13068
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Chimpanzee (100%), Mouse (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HMGA1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human HMGA1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for HMGA1 Antibody

Western Blot: HMGA1 Antibody [NBP3-13068]

Western Blot: HMGA1 Antibody [NBP3-13068]

Western Blot: HMGA1 Antibody [NBP3-13068] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (NBP3-13068) diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: HMGA1 Antibody [NBP3-13068]

Immunocytochemistry/ Immunofluorescence: HMGA1 Antibody [NBP3-13068]

Immunocytochemistry/Immunofluorescence: HMGA1 Antibody [NBP3-13068] - HMGA1 antibody detects HMGA1 protein at nucleus by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGA1 protein stained by HMGA1 antibody (NBP3-13068) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Western Blot: HMGA1 Antibody [NBP3-13068]

Western Blot: HMGA1 Antibody [NBP3-13068]

Western Blot: HMGA1 Antibody [NBP3-13068] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (NBP3-13068) diluted at 1:1000.

Applications for HMGA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGA1

HMGA1 is expected to recognise isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and isoform b (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1).

Alternate Names

high mobility group AT-hook 1, High mobility group AT-hook protein 1, High mobility group protein A1, high mobility group protein HMG-I/HMG-Y, High mobility group protein R, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, HMGA1A, HMG-I(Y), HMGIYMGC12816, HMG-R, MGC4242, MGC4854, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y

Gene Symbol

HMGA1

Additional HMGA1 Products

Product Documents for HMGA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HMGA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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