HMGCL Antibody (22H11)

Immunohistochemistry: HMGCL Antibody (22H11) [NBP3-26224] -

Immunohistochemistry: HMGCL Antibody (22H11) [NBP3-26224] - Image of HMGCL Antibody (22H11) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot: HMGCL Antibody (22H11) [NBP3-26224] -

Western Blot: HMGCL Antibody (22H11) [NBP3-26224] - Positive Western Blot detected in: Hela whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate.
All lanes: HMGCL Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 35, 27, 21 kDa
Observed band size: 35 kDa

Flow Cytometry: HMGCL Antibody (22H11) [NBP3-26224] -

Flow Cytometry: HMGCL Antibody (22H11) [NBP3-26224] - Overlay Peak curve showing Hela cells stained with HMGCL Antibody (22H11) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.