HMGCL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97971

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HMGCL Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human HMGCL fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for HMGCL Antibody

Immunocytochemistry/ Immunofluorescence: HMGCL Antibody [NBP2-97971]

Immunocytochemistry/ Immunofluorescence: HMGCL Antibody [NBP2-97971]

Immunocytochemistry/Immunofluorescence: HMGCL Antibody [NBP2-97971] - Immunofluorescence staining of HMGCL in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HMGCL polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-97971]

Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-97971]

Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-97971] - Immunochemical staining of human HMGCL in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-97971]

Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-97971]

Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-97971] - Immunochemical staining of human HMGCL in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Applications for HMGCL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGCL

The protein encoded by the HMGCL gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes thefinal step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associatedwith HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been foundfor this gene. (provided by RefSeq)

Alternate Names

3-hydroxy-3-methylglutaryl-CoA lyase, 3-hydroxymethyl-3-methylglutaryl-CoA lyase, EC 4.1.3.4, HLmitochondrial

Gene Symbol

HMGCL

Additional HMGCL Products

Product Documents for HMGCL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HMGCL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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