HNF-4 alpha/NR2A1 Antibody (10A7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26222
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10A7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human HNF-4 alpha/NR2A1 [UniProt P41235]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HNF-4 alpha/NR2A1 Antibody (10A7)
Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] -
Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Image of HNF-4 alpha/NR2A1 Antibody (10A7) diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.75% DAB.
Western Blot: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] -
Western Blot: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Positive Western Blot detected in: HL-60 whole cell lysate, Rat Liver tissue lysate.All lanes: HNF-4 alpha/NR2A1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] -
Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Staining of MCF-7 with HNF-4 alpha/NR2A1 Antibody (10A7) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 526-conjugated Goat Anti-Rabbit IgG (H+L).Applications for HNF-4 alpha/NR2A1 Antibody (10A7)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: HNF-4 alpha/NR2A1
Long Name
Hepatocyte Nuclear Factor-4, alpha
Alternate Names
HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14
Gene Symbol
HNF4A
Additional HNF-4 alpha/NR2A1 Products
Product Documents for HNF-4 alpha/NR2A1 Antibody (10A7)
Product Specific Notices for HNF-4 alpha/NR2A1 Antibody (10A7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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