HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70905
![Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2)Azide and BSA Free [NBP2-70905]](https://resources.bio-techne.com/images/products/HNF-4-alpha-NR2A1-Antibody-OTI2H2-Azide-and-BSA-Free-Western-Blot-NBP2-70905-img0001.jpg "Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2)Azide and BSA Free [NBP2-70905]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2H2
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-315 of human HNF4A(NP_849180) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free
Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2)Azide and BSA Free [NBP2-70905]
Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF-4 alpha.![Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905]](https://resources.bio-techne.com/images/products/HNF-4-alpha-NR2A1-Antibody-OTI2H2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70905-img0002.jpg "Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905]")
Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905]
Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)Applications for HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HNF-4 alpha/NR2A1
Long Name
Hepatocyte Nuclear Factor-4, alpha
Alternate Names
HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14
Gene Symbol
HNF4A
Additional HNF-4 alpha/NR2A1 Products
Product Documents for HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free
Product Specific Notices for HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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