HNF-4 alpha/NR2A1 Antibody (SR1936)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22530

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22530-100ul
NBP3-22530-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1936

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HNF-4 alpha/NR2A1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human HNF-4 alpha/NR2A1 (Uniprot #: P41235)

Specificity

Detects endogenous levels of total HNF-4 alpha/NR2A1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HNF-4 alpha/NR2A1 Antibody (SR1936)

Western Blot: HNF-4 alpha/NR2A1 Antibody (SR1936) [NBP3-22530] -

Western Blot: HNF-4 alpha/NR2A1 Antibody (SR1936) [NBP3-22530] - Western blot analysis using NBP3-22530 in HepG2 cell lysate.

Applications for HNF-4 alpha/NR2A1 Antibody (SR1936)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-4 alpha/NR2A1

HNF4 alpha is encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Gene Symbol

HNF4A

Additional HNF-4 alpha/NR2A1 Products

Product Documents for HNF-4 alpha/NR2A1 Antibody (SR1936)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF-4 alpha/NR2A1 Antibody (SR1936)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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