HNF-4 alpha/NR2A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89679

Novus Biologicals, part of Bio-Techne
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NBP1-89679
NBP1-89679-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HNF-4 alpha/NR2A1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEV

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 28827690). Rat reactivity reported in the scientific literature (PMID: 26468492).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HNF-4 alpha/NR2A1 Antibody

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679]

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining in human duodenum and tonsil tissues using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same tissues.
Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679]

Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679]

Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell lines Caco-2 and PC-3 using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: HNF-4 alpha/NR2A1 Antibody [NBP1-89679]

Immunocytochemistry/ Immunofluorescence: HNF-4 alpha/NR2A1 Antibody [NBP1-89679]

Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.
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Applications for HNF-4 alpha/NR2A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Frozen

Validated for IHC-Fr from a verified customer review.

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-89679 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-4 alpha/NR2A1

HNF4 alpha is encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Entrez Gene IDs

3172 (Human)

Gene Symbol

HNF4A

UniProt

P41235

Additional HNF-4 alpha/NR2A1 Products

Product Documents for HNF-4 alpha/NR2A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF-4 alpha/NR2A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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