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HNF-6/ONECUT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91998

Novus Biologicals, part of Bio-Techne
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NBP1-91998
NBP1-91998-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SMNNLYTPYHKDVAGMGQSLSPLSSSGLGSIHNSQQGLPHYAHPGAAMPTDKMLTPNGFEAHHPAMLGRHGEQHLTPTSAGMVPINGLPPHHPHAHLNAQGHGQLLGTAREPNPSVTGAQVSNGSNSGQMEEINTKEV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HNF-6/ONECUT1 Antibody

Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP1-91998]

Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP1-91998]

Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP1-91998] - Staining in human liver and small intestine tissues using anti-ONECUT1 antibody. Corresponding ONECUT1 RNA-seq data are presented for the same tissues.
Western Blot: HNF-6/ONECUT1 Antibody [NBP1-91998]

Western Blot: HNF-6/ONECUT1 Antibody [NBP1-91998]

Western Blot: HNF-6/ONECUT1 Antibody [NBP1-91998] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Liver tissue.
Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP1-91998]

Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP1-91998]

Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP1-91998] - Staining of human small intestine shows low expression as expected.

Applications for HNF-6/ONECUT1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-6/ONECUT1

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription

Long Name

Hepatocyte Nuclear Factor 6

Alternate Names

HNF-6A, HNF6, OC-1, ONECUT1

Gene Symbol

ONECUT1

Additional HNF-6/ONECUT1 Products

Product Documents for HNF-6/ONECUT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HNF-6/ONECUT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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