Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all HNF1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 581 to 631 of human HNF1 Homeobox A using the numbering given in entry NP_000536.5 (GeneID 6927).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HNF1 Antibody - BSA Free

Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-32126]

Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-32126]

Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-32126] - Serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- HNF1-alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: HNF1 Antibody [NBP2-32126]

Immunoprecipitation: HNF1 Antibody [NBP2-32126]

Immunoprecipitation: HNF1 Antibody [NBP2-32126] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody NBP2-32126used for IP at 6 ug/mg lysate. HNF1-alpha was also immunoprecipitated by rabbit anti-HNF1-alpha antibodies NBP2-32123, NBP2-32124, NBP2-32126. For blotting immunoprecipitated NBP2-32126 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for HNF1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 10 mcg/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HNF1

HNF1 is a transcription factor required for the expression of several liver-specific genes. The protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

HNF1 homeobox A, IDDM20, LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor, MODY3, TCF1hepatic, Transcription factor 1

Gene Symbol

HNF1A

UniProt

NP_000536.5 (Human)

Additional HNF1 Products

Product Documents for HNF1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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