Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HNF1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 581 to 631 of human HNF1 Homeobox A using the numbering given in entry NP_000536.5 (GeneID 6927).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HNF1 Antibody

Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-32126]

Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-32126]

Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-32126] - Serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- HNF1-alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: HNF1 Antibody [NBP2-32126]

Immunoprecipitation: HNF1 Antibody [NBP2-32126]

Immunoprecipitation: HNF1 Antibody [NBP2-32126] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody NBP2-32126used for IP at 6 ug/mg lysate. HNF1-alpha was also immunoprecipitated by rabbit anti-HNF1-alpha antibodies NBP2-32123, NBP2-32124, NBP2-32126. For blotting immunoprecipitated NBP2-32126 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for HNF1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 10 mcg/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HNF1

HNF1 is a transcription factor required for the expression of several liver-specific genes. The protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

HNF1 homeobox A, IDDM20, LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor, MODY3, TCF1hepatic, Transcription factor 1

Gene Symbol

HNF1A

UniProt

NP_000536.5 (Human)

Additional HNF1 Products

Product Documents for HNF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HNF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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