hnRNP A1 Antibody (4B10) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-672CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB100-672AF350

Alexa Fluor 405

NB100-672AF405

Alexa Fluor 488

NB100-672AF488

Alexa Fluor 532

NB100-672AF532

Alexa Fluor 594

NB100-672AF594

Alexa Fluor 647

NB100-672AF647

Alexa Fluor 700

NB100-672AF700

Alexa Fluor 750

NB100-672AF750

Biotin

NB100-672B

DyLight 350

NB100-672UV

DyLight 405

NB100-672V

DyLight 488

NB100-672G

DyLight 550

NB100-672R

DyLight 594

NB100-672DL594

DyLight 650

NB100-672C

DyLight 680

NB100-672FR

DyLight 755

NB100-672IR

FITC

NB100-672F

HRP

NB100-672H

Janelia Fluor 525

NB100-672JF525

Janelia Fluor 549

NB100-672JF549

Janelia Fluor 585

NB100-672JF585

Janelia Fluor 635

NB100-672JF635

Janelia Fluor 646

NB100-672JF646

Janelia Fluor 669

NB100-672JF669

mFluor Violet 450 SE

NB100-672MFV450

mFluor Violet 500 SE

NB100-672MFV500

mFluor Violet 610 SE

NB100-672MFV610

Forumulation

Catalog #

BSA Free

NB100-672

Azide and BSA Free

NBP2-80776

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Bovine, Canine

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockdown Validated, Microarray, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG_2A Clone # 4B10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all hnRNP A1 Antibodies »

Product Specifications

Immunogen

Full length partially purified human hnRNP A1. [UniProt# P09651]

Reactivity Notes

Not yet tested in other species.

Specificity

Does not react with hnRNP A2 or B

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for hnRNP A1 Antibody (4B10) [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Microarray

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: hnRNP A1

RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts.1, 3, 4 Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins of the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34 kDa up to 43 kDa.1, 3, 4 hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein binds to its RNA target through a consensus RNA-binding site UAGGGU. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein, and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP-A1 from transportin. hnRNP-A1 is ubiquitously expressed with a higher expression in proliferating and/or transformed cells than in differentiated tissues.5

Alternate Names

Helix-destabilizing protein, heterogeneous nuclear ribonucleoprotein A1, heterogeneous nuclear ribonucleoprotein A1B protein, heterogeneous nuclear ribonucleoprotein B2 protein, heterogeneous nuclear ribonucleoprotein core protein A1, hnRNP A1, hnRNP core protein A1, hnRNPA1, hnRNP-A1, HNRPA1MGC102835, nuclear ribonucleoprotein particle A1 protein, single-strand DNA-binding protein UP1, Single-strand RNA-binding protein

Gene Symbol

HNRNPA1

Additional hnRNP A1 Products

Product Documents for hnRNP A1 Antibody (4B10) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for hnRNP A1 Antibody (4B10) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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